实验室传输器或凝胶灯箱,由终身科学实验室使用,以可视化DNA,RNA或蛋白质样本通过凝胶电泳。
什么是实验室转发器?
实验室转化器含有紫外线(紫外线)或可见(blue or white)光源,玻璃观察表面,紫外线阻挡或琥珀色过滤器盖。
实验室过敏器如何工作?
的transilluminator emits UV or visible light at a specified wavelength onto the glass viewing surface (where the agarose or polyacrylamide gel is placed). Transilluminators may be used as stand-alone systems or in conjunction with gel documentation imagers.
什么是用于使用的实验室转发器?
分子生物学,基因组学,微生物学和药物开发实验室的常见,经荧光剂支持PCR段鉴定,DNA定量和样品纯化后限制酶消化。
A - Transilluminator Wavelength Channels
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凝胶转发蛋白发射紫外线(254 nm,302 nm和365 nm)或可见光(465 nm.)为了可视化荧光核酸或蛋白质样品。通过过滤液发射的UV或可见光波长必须匹配凝胶分离期间使用的荧光染料的吸光光谱。
Blue light, emitted at465 nanometers,可以最佳地查看用商业染料染色的样品,例如Sybr Green,Sybr Gold或GFP。
紫外线Visualization Wavelength Channels
254纳米光对于DNA交联是最佳的,302纳米光对于用溴化乙锭染色的短曝光凝胶是理想的,并且365纳米光最适合凝胶带切割。
B - 查看表面尺寸
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这Accuris Smart BlueCompactis a small footprint transilluminator with a 4” square viewing surface to save on benchtop space. Larger-footprint transilluminators, like UVP’s FirstLight, include a 14” x 11” viewing surface to visualize multiple gels during a single run.
C - 光源
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C1 - Ultraviolet (UV) Light Transilluminators
紫外线发射器包括A.254 nm,302 nm或365NM光激发源用于DNA,RNA或蛋白质样品的定量荧光成像。背光,25瓦UV灯在成像表面上发射高输出照明,并准确地凝胶到凝胶比较。
Blue Light vs UV Illumination - Pros & Cons
虽然UV照明导致凝胶带的更精确的凝胶带成像比视觉蓝光更精确,但UV光线带有更高的样品损伤风险(由于过度暴露)和工人损伤。所有使用紫外传递仪的操作员都必须佩戴UV面罩,并在单元运行时使用紫外线封闭表面盖。
C2 - 可见光发光剂
Light Transilluminators forNucleic Acid and Protein Gel Visualization
可见光发光剂包括A.n 8-watt bulb emitting blue light at 465 nanometers for visualization of nucleic acid or protein gels containing SYBR Green, SYBR Safe or GFP fluorescent dyes.
Visible Light vs UV Light Illuminator Differences
尽管可见光模型展示了均匀性和乐队清晰度as compared to UV systems, they carry no risk of sample damage or operator exposure. Although visible light transilluminators represent an economical alternative to UV systems, they are less effective in visualizing Ethidium Bromide gels and performing accurate gel-to-gel comparisons.
D - Transilluminator Voltage
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120伏连接适用于美国的标准实验室电源插座。
208伏或240伏连接,欧洲大陆,需要较少的电流(电流)和较小的导体,而不是设计用于120伏的设备。
E - Transilluminator Filter Cover
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E1 - Amber For Visible Light Transilluminators
琥珀色过滤器盖block blue light transmission to ensure high-quality, precise imaging of fluorescent dyes with emission wavelengths above 500 nm.
E2 - Clear, UV-Blocking For UV Transilluminators
Clear, UV-blocking filters在支持样本可视化时防止操作员曝光。UV阻挡过滤器容易调整凝胶的装载和卸载。
F - Transilluminator Light Intensity
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F1 - 单强度过敏器
Single-light transilluminators are ideal for low-throughput research settings involving gel staining with a single fluorescent dye.
F2 - Variable Intensity Transilluminators
可变光强度过敏器包括不同应用的低,中和高设置。低强度模式对于凝胶定位,多频段切除和样品成像是最佳的。中强度模式是单波段切除的理想选择。高强度模式最适合低样品浓度。
G - Special Transilluminator Features
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G1 - 智能手机兼容性
这Accuris SmartDoc 2.0system is compatible with smartphones and tablets to support the imaging of fluorescent gel stains. Each model includes an orange imaging filter to enhance sample clarity.
G2 - Double or Triple UV Light Transilluminator Sources
Analytik Jena’s UVP Benchtop Transilluminatorsinclude optional double or triple UV lamps for light emission at up to three separate, concurrent wavelengths: 254 nm, 302 nm, and 365 nm.
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在线购物各种紫外线和可见光照明器为了a wide variety of applications including general laboratory, research, PCR, DNA/RNA techniques, ELISA, protein analysis, and cell culture.
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