Life Science Labs使用了实验室透明仪或凝胶灯盒,可视化DNA,RNA或蛋白质样品通过凝胶电泳。
什么是实验室闪光照明器?
Lab transilluminators contain an ultraviolet (紫外线)或可见(blue or white) light source, glass viewing surface, UV-blocking or amber filter cover.
实验室闪光照明器如何工作?
这transilluminator emits UV or visible light at a specified wavelength onto the glass viewing surface (where the agarose or polyacrylamide gel is placed). Transilluminators may be used as stand-alone systems or in conjunction with gel documentation imagers.
实验室跨燃料量是什么?
在限制酶消化后,透光化剂在分子生物学,基因组学,微生物学和药物开发实验室中常见,支持PCR段鉴定,DNA定量和样品纯化。
A-透射仪波长通道
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凝胶透射仪发射紫外线(254 nm,302 nm和365 nm)或可见光(465 nm)用于可视化荧光核酸或蛋白质样品。透射仪发出的紫外线或可见光波长必须与凝胶分离过程中使用的荧光染料的吸光度相匹配。
Blue light, emitted at465 nanometers, is optimal for viewing samples stained with commercial dyes such as SYBR Green, SYBR Gold or GFP.
紫外线Visualization Wavelength Channels
254 nanometer light is optimal for DNA cross-linking, 302 nanometer light is ideal for short exposure gels stained with Ethidium Bromide, and 365 nanometer light is best for gel band cutting.
C-光源
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C1 - Ultraviolet (UV) Light Transilluminators
紫外线闪光灯包括254 nm, 302 nm or 365NM光激发源,用于DNA,RNA或蛋白质样品的定量荧光成像。背光25瓦紫外灯会在成像表面和准确的凝胶与凝胶比较中发出高输出照明。
蓝光与紫外线照明 - 利弊
尽管紫外线illumination results in more precise imaging of gel bands than visual blue light, UV light carries a higher risk of sample damage (due to over-exposure) and worker injury. All operators using UV transilluminators must wear UV face shields and use UV-blocking surface covers while the unit is operating.
C2-可见光透射仪
光闪光剂核酸和蛋白质凝胶可视化
可见光的透射仪在465纳米处包括8瓦灯泡发射蓝光,以可视化含有SYBR绿色,SYBR安全或GFP荧光染料的核酸或蛋白质凝胶。
可见光vs UV Light Illuminator Differences
尽管可见光模型表现出均匀性和带状清晰度的降低as compared to UV systems, they carry no risk of sample damage or operator exposure. Although visible light transilluminators represent an economical alternative to UV systems, they are less effective in visualizing Ethidium Bromide gels and performing accurate gel-to-gel comparisons.
E-透射量滤盖盖
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E1-可见光透射仪的琥珀色
琥珀色过滤器盖block blue light transmission to ensure high-quality, precise imaging of fluorescent dyes with emission wavelengths above 500 nm.
E2-清晰,紫外线闪光灯的紫外线阻滞剂
清晰,紫外线阻滞过滤器在支持样品可视化的同时,请防止操作员暴露。紫外线阻塞过滤器很容易调整凝胶的加载和卸载。
F - Transilluminator Light Intensity
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F1-单强度透射仪
单身的-light transilluminators are ideal for low-throughput research settings involving gel staining with a single fluorescent dye.
F2-可变强度透射仪
可变的光强度透射仪包括用于不同应用的低,中和高设置。低强度模式最适合凝胶定位,多个谱带切除和样品成像。中强度模式是单带切除的理想选择。高强度模式最适合低样品浓度。
G - Special Transilluminator Features
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G1-智能手机兼容性
这Accuris SmartDoc 2.0系统与智能手机和平板电脑兼容,以支持荧光凝胶污渍的成像。每个模型都包括一个橙色成像过滤器,以增强样品清晰度。
G2-双重或三重紫外线透射仪源
Analytik Jena的UVP台式式闪光照明器include optional double or triple UV lamps for light emission at up to three separate, concurrent wavelengths: 254 nm, 302 nm, and 365 nm.
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